Tetrahymena Metallothioneins Fall into Two Discrete Subfamilies
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منابع مشابه
Tetrahymena Metallothioneins Fall into Two Discrete Subfamilies
BACKGROUND Metallothioneins are ubiquitous small, cysteine-rich, multifunctional proteins which can bind heavy metals. METHODOLOGY/PRINCIPAL FINDINGS We report the results of phylogenetic and gene expression analyses that include two new Tetrahymena thermophila metallothionein genes (MTT3 and MTT5). Sequence alignments of all known Tetrahymena metallothioneins have allowed us to rationalize t...
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The expression of the heavy-metal-binding proteins, metallothioneins (MTs), are induced by heavy metals such as Cd, Cu, Zn, and Hg. MTs attenuate toxic heavy metals by sequestering them and decreasing their intracellular concentrations. However, Cr(VI) inhibits MT gene transcription in higher eukaryotes. In this study, we found that Cr affect Tetrahymena thermophila proliferation, and the letha...
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A cDNA clone for a physiologically regulated Tetrahymena cysteine protease gene was sequenced. The nucleotide sequence predicts that the clone encodes a 336-amino acid protein composed of a 19-residue N-terminal signal sequence followed by a 107-residue propeptide and a 210-residue mature protein. Comparison of the deduced amino acid sequence of the protein with those of other cysteine protease...
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The clam R. decussatus is a suspension-feeding bivalve mollusc widely distributed in European and Mediterranean coastal waters. Due to its economic importance it is extensively cultured in many countries, and particularly in Portugal where its production represents > 80 % of total shellfish production. The selection of the clam R. decussatus as a bioindicator was a consequence of its economic i...
متن کاملHints for Metal-Preference Protein Sequence Determinants: Different Metal Binding Features of the Five Tetrahymena thermophila Metallothioneins
The metal binding preference of metallothioneins (MTs) groups them in two extreme subsets, the Zn/Cd- and the Cu-thioneins. Ciliates harbor the largest MT gene/protein family reported so far, including 5 paralogs that exhibit relatively low sequence similarity, excepting MTT2 and MTT4. In Tetrahymena thermophila, three MTs (MTT1, MTT3 and MTT5) were considered Cd-thioneins and two (MTT2 and MTT...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2007
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0000291